Skip to main content
. 2002 Jun;22(11):3744–3756. doi: 10.1128/MCB.22.11.3744-3756.2002

FIG. 7.

FIG. 7.

Comparison of Mona expression in FD/Fms WT, FD/Fms Y697F, and FD/Fms Y807F cells following M-CSF stimulation. (A) Fluorescence-activated cell sorter analysis of cell morphology. Cells were either maintained in IL-3 or incubated with 2,500 U of M-CSF per ml for 3 days and then analyzed by flow cytometry for light scatter intensity to monitor cellular size (forward scatter) and cellular granularity (side scatter). (B) As for panel A, but cells were stained with May-Grünwald Giemsa reagent. (C) Induction of Mona expression in FD/Fms cells is dependent on macrophage differentiation. Cells were cultivated as indicated above for indicated times. Cell lysates from each time point were analyzed for Mona and Grb2 expression on an SDS-12% PAGE gel by immunoblotting (IB) using anti-Mona (1:1,000) or anti-Grb2 (1:5,000) antibody.