FIG. 8.

The constitutively active mutant of SHP-2 is sufficient to block myogenesis. C2C12 myoblasts were infected either with pAd-GFP (A), pAd-WT (B), or pAd-E76A (C) 24 h prior to the initiation of differentiation or with pAd-E76A 24 h postdifferentiation (D). Shown are representative photomicrographs of Wright-Giemsa-stained C2C12 myoblasts (top row) and coimmunofluorescence images of adenovirus-infected myoblasts expressing GFP that were stained with Texas red phalloidin (bottom row). Infection of C2C12 myoblasts with the pAd-E76A adenovirus 24 h prior to the initiation of differentiation prevents multinucleated myotube formation as compared to pAd-GFP- or pAd-WT-infected myoblasts. When C2C12 myoblasts are infected with pAd-E76A 24 h after the initiation of differentiation, multinucleated myotube formation proceeds normally.