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. 2002 Jun;22(11):3685–3697. doi: 10.1128/MCB.22.11.3685-3697.2002

FIG. 2.

FIG. 2.

Analysis of DACK transcripts by Northern blotting and RNA in situ hybridization. (A) About 30 μg of total RNA from Canton S adult head (lane 1), Canton S adult body (lane 2), Df(3L)C175/TM3Sb whole adults heterozygous for a deficiency predicted to remove the DACK locus (lane 3), or Canton S whole adults (lane 4) was hybridized with a DACK cDNA probe and showed a single transcript of about 4.75 kb. (B) The same blot shown in panel A was probed with an Rp49 cDNA as a loading control (53), demonstrating that DACK transcript levels are higher in the head than the body and are reduced in Df(3L)C175/TM3Sb flies relative to Canton S flies. (C) Lateral view of stage 13 wild-type embryo at the beginning of dorsal closure, showing enrichment of DACK transcripts at the leading edge of the epidermis (arrowhead). The head is to the left in this and all subsequent embryo figures.