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. 2002 Jun;22(11):3685–3697. doi: 10.1128/MCB.22.11.3685-3697.2002

FIG. 9.

FIG. 9.

JNK cascade-dependent transcription of genes at the leading edge is not affected by gains or losses of DACK function. Lateral views of embryos stained with anti-β-galactosidase antibody (A to C) or hybridized with a dpp riboprobe (D to G). Arrowheads in each panel denote the leading edge. (A) pucE69/+ embryo showing puc expression at the leading edge; (B) en-GAL4/+; pucE69, UAS-Dcdc42V12/+ embryo showing ectopic puc expression in en stripes (arrow); (C) en-GAL4/+; pucE69, UAS-DACK/+ embryo showing puc expression at the leading edge; (D) wild-type embryo showing dpp expression at the leading edge, in the lateral epidermis and in the midgut. (E) GAL4559.1/+; UAS-DACK/+ embryo showing wild-type distribution of dpp transcripts; (F) Df(3L)C175/Df(3L)C175 embryo showing dpp transcripts at the leading edge and in the lateral epidermis; (G) wild-type distribution of dpp transcripts following expression of UAS-KD-DACK by heat shock using the Hs-GAL4M-4 driver.