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Galactose-induced expression of Sup35p. The strains used were the [psi−] 628-4B transformants from one replicate of the experiment described in Table 3. Immediately (lanes 1 and 2), 2 h (lanes 3 and 4), and 6 h (lanes 5 and 6) after transfer to SG medium, cells were removed and assayed for Sup35p abundance as described in Materials and Methods. Cells are [URE3] (lanes 1, 3, and 5) and [ure-o] (lanes 2, 4, and 6)
