TABLE 5.

[URE3] stability is reduced by overexpressed SSA1 but not SSA2^a

Elevated Hsp70	Strain variant	No. of colonies				% [ure-o]
		[URE3]		[ure-o]
		With plasmid	Without plasmid	With plasmid	Without plasmid
Ssa1p	[psi−]	538	101	932	143	63
	[PSI+]	698	324	73	35	10
Ssa2p	[psi−]	579	162	0	0	0
	[PSI+]	1,663	390	48	27	3.5
None	[psi−]	1,311	282	0	0	0
	[PSI+]	336	195	4	0	0.8

^aPrimary selection plates (lacking histidine) with 628-4B transformants of pC211 (Ssa1p), pDCM64 (Ssa2p), or pRS313 (none) were replica plated onto secondary plates lacking histidine and onto USA plates. Cells from USA⁺ colonies, taken from the secondary plates lacking histidine, were grown in YPAD overnight and spread onto YPAD plates. The resulting colonies were scored for the presence of [URE3] and plasmids. Data are from 8 to 10 independently tested USA⁺ clones from each transformation.