TABLE 5.
Elevated Hsp70 | Strain variant | No. of colonies
|
% [ure-o] | |||
---|---|---|---|---|---|---|
[URE3]
|
[ure-o]
|
|||||
With plasmid | Without plasmid | With plasmid | Without plasmid | |||
Ssa1p | [psi−] | 538 | 101 | 932 | 143 | 63 |
[PSI+] | 698 | 324 | 73 | 35 | 10 | |
Ssa2p | [psi−] | 579 | 162 | 0 | 0 | 0 |
[PSI+] | 1,663 | 390 | 48 | 27 | 3.5 | |
None | [psi−] | 1,311 | 282 | 0 | 0 | 0 |
[PSI+] | 336 | 195 | 4 | 0 | 0.8 |
Primary selection plates (lacking histidine) with 628-4B transformants of pC211 (Ssa1p), pDCM64 (Ssa2p), or pRS313 (none) were replica plated onto secondary plates lacking histidine and onto USA plates. Cells from USA+ colonies, taken from the secondary plates lacking histidine, were grown in YPAD overnight and spread onto YPAD plates. The resulting colonies were scored for the presence of [URE3] and plasmids. Data are from 8 to 10 independently tested USA+ clones from each transformation.