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. 2002 Jun;22(11):3621–3632. doi: 10.1128/MCB.22.11.3621-3632.2002

FIG. 1.

FIG. 1.

Requirement for three coactivators (GRIP1, CARM1, and p300) at low levels of NR. CV-1 cells in each well of six-well culture dishes were transiently transfected with plasmids as indicated below. The transfected cells were grown with 20 nM E2 for ER or DHT for AR. Cell extracts were prepared and assayed for luciferase activity. (A) MMTV(ERE)-LUC reporter plasmid, 250 ng; 10 ng (low ER) or 100 ng (high ER) of ER expression vector; 250 ng of pSG5.HA-GRIP1, 500 ng of pSG5.HA-CARM1, and 500 ng of pCMV-p300. (B) MMTV-LUC, 250 ng; 10 to 70 ng of AR expression vector; coactivator vectors as for panel A. The graph on the right shows the activity observed with 10 ng of AR vector on an expanded scale. (C) EREII-LUC(GL45) or TK-LUC reporter plasmid, 250 ng; 1 ng of ER expression vector; coactivator vectors as for panel A. (D) EREII-LUC(GL45), 250 ng; ER expression vector, 1 ng; 500 ng of each coactivator expression vector (GRIP1, CARM1, p300, and CBP). +, present.