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. 2002 Jun;22(11):3698–3706. doi: 10.1128/MCB.22.11.3698-3706.2002

FIG. 6.

FIG. 6.

RHA-RNA polymerase II binding is not necessary for potentiation of MR AF-1 by RHA/CBP complexes. (A) Schematic representation of the RHA mutants. MTAD acts as the RNA polymerase II interacting domain. However, the point mutant RHA W339A prevents this interaction. (B) RHA-RNA polymerase II binding is not necessary for potentiation of MR AF-1 by the RHA/CBP complex. 293T cells were cotransfected with 0.25 μg of a luciferase reporter plasmid bearing progesterone response elements (PREx2-tk-luc), 50 ng of an expression vector containing pc-DNA-FLAG-MR-AF-1a or pc-DNA-FLAG-MR, and 0.3 μg of either pc-DNA-HA-RHA, pc-DNA-HA-RHAΔMTAD, pc-DNA-HA-RHAW339A, or pc-DNA-CBP in the presence (+) or absence (−) of aldosterone (10−9 M). Bars show the fold change in luciferase activity relative to the activity of FLAG-MR-AF1a (AF-1a) or FLAG-MR (MR) in the presence of aldosterone (10−9 M) without transfection of coactivators. NR, nuclear receptor.