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Northern blot analysis of kidney, liver, and brain poly(A) RNA. The blot was hybridized with an nSMase 1-specific cDNA probe and a β-actin probe for normalization. The nSMase 1-specific signal disappears in homozygous animals, but several other signals are visible, most prominently in kidney. These signals hybridized also with the neo probe, indicating splice products of the gene with the neo box. wt, wild type.
