FIG. 2.

Identification of PABP binding sites in Paip1. (A) Purified GST and GST-Paip1 proteins (∼100 ng) were resolved by SDS-10% PAGE and transferred onto a nitrocellulose membrane. The membrane was probed with a rabbit polyclonal anti-GST antibody. Positions of molecular mass markers are shown on the left. wt, wild-type. (B) Autoradiography of a duplicate membrane processed for far-Western analysis with ³²P-labeled HMK-PABP as a probe. (C) Schematic diagram of the results from the interaction of GST-Paip1 fragments with PABP. Relative binding was evaluated visually. Light gray, dark gray, and black boxes represent GST, PAM2, and PAM1, respectively.