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. 2002 Jun;22(11):3769–3782. doi: 10.1128/MCB.22.11.3769-3782.2002

FIG. 8.

FIG. 8.

Stoichiometry of the PABP-Paip1 interaction determined by SPR. (A) Interactions between PABP(RRM1-4) and Paip1 or Paip2 captured on a PABP surface. Paip1 (1 μM) or Paip2 (100 nM) was injected over a PABP surface (1,500 RU) and a mock surface. The Paip1 and Paip2 injections were followed by an injection of PABP(RRM1-4) (100 nM) (dashed line). (B and C) Enlargements of PABP(RRM1-4) binding curves shown in panel A after injection of PABP(RRM1-4) for Paip1 (B) or Paip2 (C). (D) PABP(C-term) interactions with Paip1-PABP(RRM1-4) complex. Paip1 (25 nM) preincubated (or not) with PABP(RRM1-4) (75 nM) was injected (300 s) over a PABP(C-term) surface (150 RU) and a mock surface.