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. 2002 Jun;22(12):4167–4180. doi: 10.1128/MCB.22.12.4167-4180.2002

FIG. 3.

FIG. 3.

Enzymatic activity of Sir2 is required for association of Sir2, Sir3, and Sir4 proteins with telomeric DNA regions and the HML mating type locus. (A and B) Chromatin immunoprecipitation was carried out from a sir2 deletion strain (sir2Δ), a SIR2 wild-type strain (SIR2+), and strains containing sir2 alleles that encode enzymatically inactive Sir2 proteins (sir2-H364Y and sir2-G262A) with anti-Sir2, anti-Sir3, and anti-Sir4 antibodies. Panels show phosphorimager data of PCR amplifications corresponding to input (WCL) and immunoprecipitated chromatin for the indicated regions of the VI-R telomere and the HML locus. The following strains were used: DMY1865 (sir2Δ), DMY1866 (SIR2+), DMY1865 (sir2-H364Y), and DMY1867 (sir2-G262A). See Fig. 2A for locations of primers.