TABLE 2.
Gcn5p is required for repression of ARG1 in rich mediuma
| Strain | GCN5 plasmid | β-Galactosidase activity
|
|
|---|---|---|---|
| LacZ unitsb | Ratio (FY1370/FY86) | ||
| FY86 | None | 7.9 ± 0.4 | |
| FY1370 | None | 44.2 ± 0.6 | 5.6 |
| FY1370 | Myc-Gcn5p | 12.6 ± 0.3 | 1.6 |
| FY1370 | Myc-Gcn5pE173Q | 43.1 ± 0.6 | 5.5 |
a
Saturated cultures of FY86 (wild type), FY1370 (gcn5), FY1370 expressing Myc-GCN5, and FY1370 expressing Myc-GCN5E173Q, each containing YCp87-ARG1-lacZ, were grown in minimal medium and then diluted 1/100 into YPD and grown to an OD600 of approximately 1.5.
b
β-Galactosidase activity was determined using ONPG as the substrate, standardizing to cell density. Numbers represent the means and standard errors of the means from 10 independent experiments.