Fig. 1. MT bands are kept at a constant position from the growing zygote tip.
a Time-lapse images of a zygote expressing MT (green) and nuclear (magenta) markers. Maximum-intensity projection (MIP) images of two-photon excitation microscopy (2PEM) images are shown. Numbers indicate the time (hour:min) from the first frame. White and yellow brackets show the subapical MT band and PPB, respectively. The yellow arrowhead indicates the cell division plane. b Schematic illustration of the method used for image quantification. For details, see “Methods”. c Time course of cell growth rate (dL/dt; red) and tip radius (re; blue) of the zygote shown in (a). d Contour dynamics of the zygote up to just after cell division (17:20), with the time indicated by color code. e Kymograph showing MT and nuclear signal intensities, which are projected onto the zygote centerline. Background areas above the zygote are shown to indicate the apical end of the cell. Dashed black lines indicate the upper and lower ends of the MT band, as determined by Gaussian fitting (as shown in (b) and Supplementary Movie 1). The yellow arrow marks the timing of cell division. Images are representative of 14 samples. Scale bars: 10 μm. Source data are provided as a Source Data file.