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. 2026 May 22;17:6752. doi: 10.1038/s41467-026-72280-4

Fig. 5. The MT band disappears after zygote elongation ceases.

Fig. 5

a–f Dynamics of wild-type (WT, a–c) and yda (d–f) zygotes expressing MT (green)/nuclear (magenta) markers. a, d Time-lapse images. White and yellow brackets show the MT band and PPB, respectively. Yellow arrowheads indicate the cell division plane. b, e Time course of cell growth rate (dL/dt; red) and tip radius (re; blue). c, f Kymographs showing MT and nuclear signal intensities. Dashed black lines indicate the MT band based on Gaussian fitting. The fitting of yda shows the disappearance of the subapical MT band after 05:20 and the appearance of the PPB at 10:40 (Supplementary Movie 8). Note that the nuclear signal was faint and barely detectable. Yellow arrows mark the timing of cell division. g–l Results of parameter estimation (g–i) and comparisons of tension and MT band pattern (j–l) for the yda zygote shown in (d). g Comparisons of cell growth rate (dL/dt) and cell tip radius (re) between time-lapse observation data (Data; magenta) and reconstructed model zygote (Model; green). h Estimated turgor pressure (P) and extensibility range (lg). i Comparisons of the contour dynamics of Data and Model. j–l Calculated circumferential surface tension (σθ) (j), its time change (Dσθ/Dt) (k), and MT signal intensity (l) (Supplementary Movie 9). Magenta lines and crosses in (l) indicate the MT bands and their centers, respectively. Magenta and orange arrowheads indicate when the large time change of surface tension is lost (03:20) and when the MT bands are no longer detectable (05:40), respectively. Images are representative of 14 and 12 samples for WT and yda, respectively. m Boxplot showing the ratio of apical to basal cell length. Center line, median; box limits, upper and lower quartiles; whiskers, maximum and minimum points within 1.5 × the interquartile range from box limits. Individual data points are also shown. Significant difference was detected by two-sided Student’s t-test (**P = 2.17 × 10−9; n = 14 and 12 for WT and yda, respectively). WT zygotes correspond to “none” in Fig. 2m. Scale bars: 10 μm. Source data are provided as a Source Data file.