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. 2002 Jun;22(12):4419–4432. doi: 10.1128/MCB.22.12.4419-4432.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 4. — Chk2 phosphorylates the SCD in vitro. In vitro kinase assays were performed on soluble GST-Chk2 and mutants in the presence of the substrate GST-HA-SCD or GST-HA-SCD(7A) and [γ−³²P]ATP. The top panel shows [γ-³²P]ATP incorporation into GST-HA-SCD or GST-HA-SCD(7A). GST-HA-SCD or GST-HA-SCD(7A) (middle panel). GST-Chk2 and its mutants (bottom panel) were detected by immunoblotting with anti-GST. IB, immunoblotting.