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. 2002 Jun;22(12):4020–4032. doi: 10.1128/MCB.22.12.4020-4032.2002

FIG. 1.

FIG. 1.

Coimmunoprecipitation of BRCA1 and c-Abl. (A) Positions of the epitope domains of the anti-BRCA1 and the anti-c-Abl antibodies used in this study. The MS110 and SG11 antibodies recognize the N-terminal and the C-terminal domain of BRCA1, respectively; the Ab-1 and Ab-3 antibodies recognize the kinase-adjacent domain and the C-terminal domain of c-Abl, respectively. (B) Nuclear extracts from exponentially growing 293 cells were subjected to BRCA1 and c-Abl immunoprecipitation (IP). The immunoprecipitates were analyzed by immunoblotting (IB). The masses of endogenous BRCA1 and c-Abl are 220 and 140 kDa, respectively. αBRCA1, anti-BRCA1 antibody; αc-Abl, anti-c-Abl antibody. (C) Nuclear extracts from exponentially growing HCC1937 cells were subjected to BRCA1 and c-Abl immunoprecipitation.