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. 2002 Jun;22(12):4020–4032. doi: 10.1128/MCB.22.12.4020-4032.2002

FIG. 3.

FIG. 3.

Disruption of the BRCA1-c-Abl complex after irradiation. Control and irradiated (20 Gy) 293 cells were collected at the indicated times. (A) Nuclear extracts were subjected to BRCA1 and c-Abl immunoblotting (IB) with MS110 and Ab-3 antibodies, respectively. λ-PPase, λ-phosphatase. (B) The same batch of extracts was subjected to BRCA1 and c-Abl immunoprecipitation (IP) with MS110 and Ab-3 antibodies, respectively. Preimmune mouse IgGs were used as controls. αc-Abl, anti-c-Abl; αBRCA1, anti-BRCA1. (C) As described in the legend to Fig. 2, purified GST-BRCA1#6, GST-SH3+SH2+kinase, GST-SH3, and GST fusion proteins were incubated with nuclear extracts from irradiated 293 cells; GST adsorbates were collected and subjected to BRCA1 or c-Abl immunoblotting using the same antibodies used for Fig. 2.