FIG. 2.

HMGB1 potentiates Rta-mediated activation of BHLF-1 in a cell-based assay; 50 ng of BHLF-1 luciferase reporter (pHLlux) was transfected into BHK-21 cells, along with 5 or 50 ng of Rta effector plasmid (pSV40Rta) in the absence (white bars) or presence of 1 μg of HMGB1 expressor (pSV40HMGB1; grey bars). After 24 h the cells were lysed, and luciferase activity was measured. Transfections were performed in triplicate, and the standard deviation is indicated by error bars. The highest luciferase value (1.5 million light units on average) was assigned an arbitrary value of 100%, and all other values were normalized to that.