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Chromatin immunoprecipitation of the CYP1A1 enhancer region. PCR amplification of the CYP1A1 enhancer by affinity-purified antibodies raised against SRC-1, NCoA-2, and p/CIP. Total input DNA for each PCR can be visualized in the first two lanes of each panel. Precipitation (I.P.) reactions performed in the absence and presence of TCDD are shown in lanes 3 and 4 of each panel, respectively. XRE's, xenobiotic compound-responsive elements.
