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. 2002 Jun;22(12):4319–4333. doi: 10.1128/MCB.22.12.4319-4333.2002

FIG. 9.

FIG. 9.

FIG. 9.

FIG. 9.

Identification of SRC-1 interaction domains within ARNT with the yeast two-hybrid and GST pulldown assays. (A) Schematic of the mARNT mutants used in the GST pulldown assay. Mutants were cloned into pcDNAI/Neo or pcDNA3.1/His C. Critical amino acids deleted or included are indicated in parentheses. (B) GST pulldown of ARNT minimal mutants by GST-SRC-1763-1100. Complexes were fractionated by SDS-PAGE on gels ranging in acrylamide concentration from 7.5 to 10%. (C) Effect of the loss of the ARNT TAD and helix 2 on recruitment of SRC-1 in the ARNT-dependent luciferase assay. Above appears a schematic of mARNT deletion mutants fused to the GAL4 DBD.