FIG. 3.

Lack of an essential subdomain of FOG-1 required for terminal erythroid maturation of the FOG-1^−/− cell line (excluding a GATA interacting domain). A schematic representation of each construct is shown next to the percentage of benzidine-positive cells obtained from at least two independent rescue experiments and displayed as the mean value ± the standard error of the mean. Three regions of high sequence conservation (outside of the zinc fingers) between FOG-1 and other members of the FOG family are indicated above the top of the wild-type construct as CR1, CR2, and CR3. Darker shaded boxes represent GATA-binding zinc fingers, and black diagonal lines represent substitution of alanine for the tyrosine residue immediately preceding the final cysteine residue of the zinc finger.