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. 2002 Jun;22(12):4241–4255. doi: 10.1128/MCB.22.12.4241-4255.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 2. — SLAP-2 expression in murine tissues and cell lines. (a) Generation of SLAP-2-specific antisera. Rabbit antisera generated against a GST fusion protein containing the murine SLAP-2 carboxy terminus (anti-SLAP-2-C) were tested against transfected MYC-tagged SLAP-2 (crude antibody) and endogenous SLAP-2 (affinity-purified antibody). IgH, immunoglobulin heavy chain; IP, immunoprecipitation; RαM, rabbit anti-mouse IgG. (b) Expression of SLAP-2 in murine tissues. One milligram of total protein lysate isolated from the tissues of an 8-week-old mouse was immunoprecipitated and immunoblotted with crude anti-SLAP-2-C antibody. sk., skeletal. (c) Expression of SLAP-2 in murine cell lines. One milligram of total protein lysate from various hematopoietic and nonhematopoietic cell lines was immunoprecipitated and immunoblotted with affinity-purified anti-SLAP-2-C antibody.