FIG. 3.

The SH3 domain of c-Src is important for the interaction with and phosphorylation of hnRNP K. HeLa cells were transfected with His-hnRNP K and c-Src, Src(−SH3), or Src(−SH2) expression vectors. The products of a His immunoprecipitation (A and B) or a Src immunoprecipitation (C and D) were analyzed after SDS-PAGE in Western blot assays using antibodies against the His-tag to detect the precipitated His-hnRNP K (A) and p-Tyr to examine Tyr phosphorylation of hnRNP K (B). After Src immunoprecipitation, c-Src expression was assessed with an anti-c-Src antibody (C) and the coprecipitated His-hnRNP K was detected with an anti-His antibody (D).