TABLE 1.
Extent of deletions and complementarity at the recombination sites in G418r/Ganr subclones
| Cell line | Subclone | Deletiona | Complem entarity (bp)b |
|---|---|---|---|
| A211 | FS-1 | 17 bp/3.0 kb | 4 |
| FS-2 | 8 bp/2.6 kb | 1 | |
| A405 | FS-3 | 0.5 kb/1.8 kb | 4 |
| FS-4 | 1.0 kb/1.0 kb | ND | |
| EJ-30 | G55 | ≥0 bp | ND |
| G71 | ≥1.3 kb | 1/0c | |
| G45 | ≥9.8 kb | 3 | |
| G65 | ≥8.7 kb | 1 |
a
The distance from the recombination site to the I-SceI site in the A211 and A405 subclones and/or the distance between the sequences at the recombination site in the EJ-30 subclones is indicated. If we assume a single event initiated recombination in the EJ-30 subclones, the difference in the location in the plasmids would represent the minimum amount of degradation.
b
Complementarity at the recombination site. The recombination site in some subclones could not be sequenced (ND = not determined), apparently due to the presence of a perfect inverted repeat.
c
Complementarity on either end of a 187-bp insert of human DNA.