FIG. 2.

Expression pattern of the Mcad/lacZ mutant allele in embryonic and adult mice. Whole-mount preparations of heterozygous animals from E8.5 to E13 (A to E), sagittal section of the eye of an E13.5 embryo (F), and sagittal sections of adult eyes (G and H) and adult brains (I and J) were stained for β-galactosidase activity. Blue cells indicate expression of the mutant allele in head mesenchyme (A), in myotomes and individual muscles (B to E), and in nuclei of lens fibers during embryonic development (F). Cryotome sections of isolated eyes (G and H) and brains (I and J) of adult animals show expression in a distinct cell layer of the neuroretina, gyrus dentatus of the hippocampus (I), and undefined single dispersed cells of the brain stem (J). l, lens; r, retina; nr, neuroretina; pc, pigment cells; gd, gyrus dentatus.