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Activation of full-length MTK1 (MTK1-FL) by GADD45 proteins. Yeast strain FP75 was transformed with two expression plasmids (p414TEF for MTK1-FL and p426GAL for GADD45) with (+) or without (−) the indicated cDNA sequences. p426GAL is a multicopy plasmid with the galactose-inducible GAL1 promoter. Transformed cells were spread on YPGal agar plates supplemented with (+) or without (−) 1.5 M sorbitol.
