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Three-hybrid interaction analysis. Yeast L40 cells were transformed with three plasmids (ACT-MTK1, DB-MKK6-K/A, and GADD45 constructs) as indicated. A minus sign indicates the corresponding vector (pACTII, pBTM118, or pRS422-THL) without an insert; a plus sign indicates the vector with an insert. Transformed cells were spread on the appropriate synthetic agar plates supplemented with (+His) or without (−His) histidine.
