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Integrin ligation specifically down-regulates p21CIP1 and p27KIP1. (A) ECV 304 cells were plated on immobilized anti-β1 (P4C10), anti-αvβ3 (LM609), or anti-α5β1 (JBS5) for 30 min and photographed with a 20× objective. (B) ECV 304 cells attached to immobilized anti-β1, anti-αvβ3, or anti-α5β1 MAb were analyzed for p21CIP1, p27KIP1, and actin protein levels by Western blotting.
