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. 2002 Jul;22(13):4587–4597. doi: 10.1128/MCB.22.13.4587-4597.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 3. — Cell attachment to FN affects p21^CIP1 protein stability. (A) Total mRNA isolated from ECV 304 cells previously plated on FN for the indicated times was analyzed for p21^CIP1 mRNA levels by Northern blotting. β-Actin mRNA levels were measured as a loading control. (B) ECV 304 cells transiently transfected with HA-tagged p21^CIP1 were allowed to attach to FN or suspended upon BSA, and then HA-p21^CIP1 levels were analyzed by Western blotting. Actin levels were used as a loading control. (C) ECV 304 cells transfected with HA-p21^CIP1 were plated on FN or kept in suspension (BSA), and then the stability of HA-p21^CIP1 was detected by pulse-chase analysis with incorporated [³⁵S]methionine. The values shown are the means of two or three independent experiments.