FIG. 2.
Genotyping of wild-type, heterozygous, and AI-deficient mice by long-range PCR. Reaction A (lanes 1) contains two AI-specific primers, one located within the targeted region (MAIF/I3) and one outside the targeted region of the AI gene [MAIR3′UTR(∗+59)]. Reaction B (lanes 2) contains a NeoR gene-specific primer [NeoF(412)] and an AI-specific primer located outside the targeted region [MAIR3′UTR(∗+59)]. For location of the primers on the wild-type and modified AI genes, see Fig. 1. As expected, wild-type mice exhibited a 2.5-kb band in reaction A but no product in reaction B; heterozygous mice exhibited a 2.5-kb and a 4.0-kb (often very weak or absent) band in reaction A and a 3.0-kb band in reaction B; and AI-deficient mice exhibited a strong 4.0-kb band in reaction A and a 3.0-kb band in reaction B.