Table 1.

Characterization and validation.

Classification	Test	Result	Data
Morphology	Photography	Normal iPSC-like morphology	Fig. 1 Panel A
Phenotype	Quantitative analysis: RT-qPCR	Positive expression of undifferentiated iPSC state markers Nanog, Sox2, and Oct3/4 in the two DM1 lines and absent in differentiated CMs	Fig. 1 Panel B
	Qualitative analysis: Immunofluorescence staining	Positive expression of Nanog, Sox2, and Oct3/4 in both lines	Fig. 1 Panel C
Genotype	Whole genome array (KaryoStat™ Assay) Resolution 1–2 Mb]	Normal karyotype: 46, XX for both lines	Fig. 1 Panel E
Identity	Microsatellite PCR (mPCR) or	N/A	N/A
	STR analysis	Exact match	Submitted to an archive with a journal
Mutation analysis (IF APPLICABLE)	Southern blot or WGS	N/A	N/A
	Southern blot or WGS	N/A	N/A
Microbiology and virology	Mycoplasma	Mycoplasma testing by luminescence: Negative	Fig. 1 Panel F
Differentiation potential	Directed differentiation	Positive IF staining of three germ layer markers	Fig. 1 Panel D
List of recommended germ layer markers	Expression of these markers has to be demonstrated at mRNA(RT-qPCR) or protein (IF) levels, at least 2 markers need to be shown per germ layer	Ectoderm: Pax6, Otx2 Endoderm: Sox17, Foxa2 Mesoderm: Brachyury, Tbx6	Fig. 1 Panel D
Donor screening (OPTIONAL)	HIV 1 + 2, Hepatitis B, Hepatitis C	N/A	N/A
Genotype additional info (OPTIONAL)	Blood group genotyping	N/A	N/A
	HLA tissue typing	N/A	N/A