Table 2.

Reagents details.

Antibodies and Primers for Immunocytochemistry, Flow Cytometry, and PCR
Marker	Antibody	Dilution	Company (Cat #)	RRID
Undifferentiated iPSC state marker	Rabbit Anti-Nanog	1:200	Proteintech (142951–1-AP)	AB_1607719
Undifferentiated iPSC state marker	Mouse IgG2bκ Anti-Oct-3/4	1:200	Santa Cruz Biotechnology (Sc-5279)	AB_628051
Undifferentiated iPSC state marker	Mouse IgG1κ Anti-Sox2	1:200	Santa Cruz Biotechnology (Sc-365823)	AB_10842165
Differentiation marker: Ectoderm	Goat Anti-Otx2	1:200	R&D Systems (963273)	AB_2157172
Differentiation marker: Ectoderm	Rabbit Anti-Pax6	1:100	Thermo Fisher Scientific (426600)	AB_2533534
Differentiation marker: Endoderm	Goat Anti-Sox17	1:200	R&D Systems (963121)	AB_355060
Differentiation marker: Endoderm	Rabbit Anti-Foxa2	1:250	Thermo Fisher Scientific (701698)	AB_2576439
Differentiation marker: Mesoderm	Goat Anti-Brachyury	1:200	R&D Systems (963427)	AB_2200235
Differentiation marker: Mesoderm	Rabbit Anti-Tbx6	1:200	Thermo Fisher Scientific (PA5-35102)	AB_2552412
Secondary	Alexa Fluor 488 Goat Anti-Mouse IgG1	1:1000	Thermo Fisher Scientific (A-21121)	AB_2535764
Secondary	Alexa Fluor 488 Donkey Anti-Goat IgG (H + L)	1:1000	Thermo Fisher Scientific (A-11055)	AB_2534102
Secondary	Alexa Fluor 555 GoatAnti-Rabbit IgG (H + L)	1:500	Thermo Fisher Scientific (A-21428)	AB_2535849
Secondary	Alexa Fluor 555 Donkey Anti-Rabbit IgG (H + L)	1:500	Thermo Fisher Scientific (A-31572)	AB_162543
Secondary	Alexa Fluor 647 Goat Anti-Mouse IgG2b	1:250	Thermo Fisher Scientific (A-21242)	AB_2535811
Primers for PCR and qPCR
Target	Size of Band	Forward/Reverse primer (5′–3′)
Sendai virus genome (qPCR)	181 bp	Mr04269880_mr
GAPDH (qPCR)	471 bp	Hs02786624_g1
SOX2 (qPCR)	258 bp	Hs04234836_s1
NANOG (qPCR)	327 bp	Hs02387400_g1