FIG. 3.
Motif C mutants are sensitive to MMS but not to HU, UV, or ionizing radiation. dfp1+ (GBY572), dfp11-459 (AFY6), dfp11-376 (AFY7), cds1Δ (GBY180), rad1Δ (GBY190), and rad3-h1.5 (FY382) were treated with HU (A), UV (B), or ionizing radiation (C) as described in Materials and Methods. Viability relative to that of the untreated culture is plotted. (D) dfp1+ (GBY572), dfp11-459 (AFY6), dfp11-376 (AFY7), and cds1Δ (GBY180) were treated with 0.03% MMS as described in Materials and Methods. Viability relative to that of the untreated culture is plotted. Points represent the means of the results of three experiments, and error bars span 1 standard deviation. (E) The region of the C terminus of dfp1+ that confers MMS resistance was mapped by treating dfp1+ (GBY572), dfp1Δ497-505 (AFY23), dfp1Δ460-494 (AFY16), dfp11-494 (AFY22), and dfp11-459 (AFY6) with MMS and plotting their viabilities relative to that of the untreated culture. A representative example of the results of two similar experiments is displayed.