FIG. 2.
(A) Effects of UVB irradiation on cell cycle and apoptosis markers in HEKn and HEKn-E6/E7. Cells grown in BKM+exoGF were irradiated with the indicated doses of UVB, and the expression of the indicated proteins was determined in immunoblot analyses 21 h postirradiation. PARP, poly(ADP)ribose polymerase. (B) Inhibition of ERK activity by UVB and anisomycin. HEKn (grown in BKM+exoGF) were treated with UVB (1,200 J/m2) or anisomycin (10 μg/ml). ERK activity was assessed 30 min later in immunocomplex kinase assays (see Materials and Methods). Lane 4 of the boxed insert shows the control immunoprecipitation (IP) with an irrelevant antibody. Error bars represent the standard deviations from experimental points in triplicates.