FIG. 1.

Rapamycin-sensitive gene expression in human BJAB cells. (A) At all time points rapamycin eliminates the phosphorylation of threonine 389 of S6K1. EtOH, ethyl alcohol. (B) Representation of k means cluster analysis of 543 genes regulated at 0, 15, 30, 60, and 120 min and 12 and 24 h after addition of 20 nM rapamycin. Genes are represented vertically, and experimental conditions (i.e., rapamycin time course) are displayed horizontally. Fold changes are indicated colorimetrically. (C) Quantitative RT-PCR analysis confirms rapamycin-induced gene expression changes detected using microarrays. Expression changes in four genes (BCKAD E1 alpha in cluster C, ASS in cluster D, FAS in cluster G, and SREBP-1 in cluster H) were determined in RNA samples prepared independently from those used for microarray analysis. The standard deviation for each time point was calculated from the triplicate samples in RT-PCR.