FIG. 4.

Effects of rapamycin treatment on nutrient metabolism and cell proliferation in murine CTLL-2 T lymphocytes. All nutrient measurements were performed over a 48-h period in triplicate as described in Materials and Methods. (A) Cells treated with rapamycin increased the usage of glutamine by 54% (P < 0.01). (B) Cells treated with rapamycin increased the production of ammonia when cultured in media with normal (2 mM) or low (0.1 mM) concentrations of glutamine by 44% (P < 0.01) or 20% (P < 0.05), respectively. (C) Cells treated with rapamycin increased the consumption of glucose by 19% (P < 0.05) and also increased lactate production by 31% (P < 0.01). (D) CTLL-2 cells (starting at 10⁴/ml) were grown in control medium with or without rapamycin (20 nM), medium with 0.1 mM glutamine with or without rapamycin (20 nM), or medium with 0.3 mM glucose with or without rapamycin (20 nM) for 72 h. Triplicate samples were counted every 24 h.