FIG. 7.

TAPP1 and TAPP2 show delayed and sustained kinetics of PH domain-mediated membrane recruitment after BCR cross-linking. BJAB cells were transiently transfected with the indicated PH domain-EGFP fusion and activated by BCR cross-linking. (A) Confocal images were acquired at 30-s intervals after activation. Note the marked differences in the kinetics of PH domain-mediated translocation events. (B) The indicated transfectants were stimulated by BCR cross-linking and imaged after 30 or 60 min. Individual representative cells are shown, while a quantitative analysis over multiple cells is shown in Fig. 8.