FIG. 2.

Pre- and postnatal growth retardation and eyelid abnormalities in mice lacking Pref-1. (A) Intrauterine growth retardation. Pref-1-null embryos are smaller than wild-type embryos at E18.5. The embryos were generated from crossbreeding Pref-1 heterozygotes (+/−). (B) Blepharophimosis. Anterior view of mice at age 27 days showing the left or both eyelid defects observed on the BALB/cJ strain background. (C) Skeletal malformation. Arrows indicate asymmetric zigzagged sternums and fusion of ribs in Pref-1-null mice. (D) Growth retardation in Pref-1-null mice of the C57BL/6J background. Female and male wild-type and Pref-1-null mice (n = 27 to 37 per group) were fed high-fat (45 kcal%) diet ad libitum from 21 days old and were weighed at 4-day intervals. Statistics were performed with a two-tailed t test. For female mice, from day 21 to day 68, P < 0.01, and from day 72 to day 76, P < 0.05. For male mice, from day 21 to day 52, P < 0.01.