FIG. 3.

Accelerated adiposity in mice lacking Pref-1. (A) Percentage of fat pad and organ weight relative to body weight. Fat depots and organs were dissected from 16-week-old wild-type (WT) and Pref-1-null mice fed a high-fat diet (n = 7 to 10 per group). Ing, inguinal fat pad; Epi, epididymal fat pad; Retro, retroperitoneal fat pad; Para., parametrial fat pad; BAT, brown adipose tissue. All values are means ± standard errors. ∗, P < 0.05; ∗∗, P < 0.01. (B) Paraffin section of inguinal fat pad from 16-week-old female mice and the distribution of fat cell size. The size of at least 300 cells per sample (mean of four mice per group) was determined with NIH Image software. The distribution of cell volume is shown. The scale bar represents 50 μm. (C) Expression of adipocyte marker genes in adipose tissues and liver. Total RNA was extracted from adipose tissue of 16-week-old wild-type (W) and Pref-1-null (N) mice fed a high-fat diet.