FIG. 4.

Mice with maternal or paternal inheritance of the pref-1-knockout allele (m−/+ or +/p−) have phenotypes similar to those of wild-type or null mice, respectively. (A) Pref-1 protein expression in E17.5 embryos generated from matings of male heterozygotes (+/−) and female wild-type mice or vice versa. Pref-1 protein is expressed in m−/+ heterozygotes, but not in +/p− heterozygotes. (B) Body weights of F₁ littermates were measured at 21 and 56 days of age. Values are means ± standard errors with n = 5 to 13 per group. ∗, P < 0.05; ∗∗, P < 0.01. (C) Percentage of fat pad mass (total white fat, including inguinal, retroperitoneal, and reproductive fat) relative to body weight (16 weeks old; n = 7 to 9 per group). All values are means ± standard errors. ∗, P < 0.05. Statistics were performed with a two-tailed t test for panels B and C.