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. 2002 Aug;22(15):5626–5637. doi: 10.1128/MCB.22.15.5626-5637.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 4. — Direct physical interaction between HNF-4 and TRAP/SMCC/Mediator. (A) Schematic representation of full-length HNF-4 and various derivatives used in GST interaction assays (see text for details). Relevant features only are shown. DBD, DNA binding domain; P-rich, proline-rich; AF-1 and AF-2, activation functions 1 and 2, respectively. (B) Purified TRAP/SMCC/Mediator (10% input, lane 1) was incubated with glutathione-Sepharose beads containing GST (lane 2), GST-HNF-4ΔC1 (lane 3), or GST-HNF-4ΔC2 (lane 4). After being washed to remove unbound material, the resins were eluted with Sarkosyl and analyzed by immunoblotting following SDS-PAGE.