FIG. 8.

Role of LXRα in adipocyte metabolism. Fully differentiated 3T3-L1 cells that expressed ectopic LXRα were incubated in the absence (Con) or presence of 1 μM T0901317 for 24 h. (A) Cells were labeled with [¹⁴C]glucose, and basal glucose uptake measurements were performed. (B) Lysates were analyzed by immunoblot for GLUT1. (C) Cells were labeled with [¹⁴C]acetate, and glycogen synthesis was measured. (D) NEFA and glycerol efflux from the cells into the medium was determined. (E) Cells were labeled with [¹⁴C]acetate, and lipids were extracted from the medium (top) or cell lysates (bottom) and separated by thin-layer chromatography. The origin, phospholipids (PL), cholesterol, diacylglycerol (DAG), and triacylglycerol (TAG) are indicated. These experiments are representative of at least three independent experiments.