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Purification of tetrameric and trimeric complexes of pol-prim and the p68 subunit. Purified preparations of the pol-prim tetramer, trimer, and p68-his were analyzed by SDS-PAGE and Coomassie blue staining (A), or by immunoblotting (B) with two p180-specific antibodies (left panel), a p68-specific antibody (center panel), or a mixture of all three antibodies (right panel). The positions of marker proteins of known size are indicated at the right in kilodaltons.
