TABLE 1.
Ability of mutated GBD-arg80 proteins to interact with Arg81 and Mcm1a
| Hybrid | β-Galactosidase sp act (nmol of o-nitrophenyl-β-d-galactopyranoside hydrolyzed/min/mg of protein)
|
||
|---|---|---|---|
| GAD (pACTII) | GAD-Arg81 (pME9) | GAD-Mcm1 (pME19) | |
| GBD-Arg80 (pME46) | <1 | 89 | 4 |
| GBD-arg80Y87F-Y110F (pAJ257) | <1 | 129 | 2 |
| GBD-arg80Y110F-βI swap-T135S (pAJ256) | <1 | 31 | <1 |
| GBD-arg80Y110F-T135S (pAJ255) | <1 | 61 | <1 |
a
Transcription activation of the lacZ gene was estimated by determination of β-galactosidase activity in S. cerevisiae strain HY cotransformed with plasmids expressing GAD (pACTII), GAD-Arg81 (pME9), or GAD-Mcm1 (pME19) and the different wild-type or mutated GBD-arg80 fusions. Values shown are the means of three independent measurements with variations of less than 15%.