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Actin content of limbs from wild-type (+/+), hemizygous (+/−), and homozygous (−/−) null mice 3 days after birth. Three separate samples were assayed by immunoblotting. Total actin levels were assessed with MAb C4, vascular actin levels were determined with MAb 1A4, and striated actins (skeletal actin plus cardiac actin) were measured with MAb 5C5, as described in Materials and Methods.
