FIG. 9.

TRRAP expression results in increased acetylation of histones H3 and H4 at the mdm2 promoter. 293 cells were transfected with the murine mdm2 promoter luciferase construct and a CMV-driven TRRAP expression vector (or empty vector), as indicated. ChIP analysis was then performed as for Fig. 6c using antibodies against TRRAP (right) or acetylated forms of histone H3 (left) or H4 (middle). The ChIP PCR products generated from the transfected mdm2 promoter were resolved by agarose gel electrophoresis, stained with ethidium bromide, quantitated, and normalized to input DNA. The resulting values were expressed graphically.