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. 2002 Sep;22(17):6100–6110. doi: 10.1128/MCB.22.17.6100-6110.2002

FIG. 2.

FIG. 2.

Dkk2, but not Dkk1, synergizes with LRP6 to activate β-catenin signaling pathways. (A and B) Embryos were injected ventro-vegetally with LRP6 mRNA (3 ng) and Dkk1 (5 pg) or Dkk2 (50 pg) mRNAs, as shown. (A) Morphology of injected embryos. (B) Combined results of three separate experiments. (C) Activation of the Siamois promoter by coexpression of LRP6 and Dkk1 or Dkk2. Embryos were injected into two ventral-animal blastomeres with pSia-Luc plasmid (20 pg) and with the following RNAs: LRP6 (5 ng), Dkk1 (20 pg), or Dkk2 (50 pg), as indicated. −, no RNA was coinjected with the pSia-Luc plasmid. (D) Dkk2 inhibits Wnt8, but synergizes with LRP6. Embryos were injected with pSia-Luc plasmid (20 pg), Wnt8 (1 pg), LRP6 (2 ng), and Dkk2 (50 pg), as indicated. (C and D) Luciferase activity was measured in embryonic lysates at stages 10 to 10+. Bars depict the means ± standard errors of triplicate samples, containing five embryos each. Each experiment was performed at least three times; a representative experiment is shown.