FIG. 5.

Defective biogenesis of Tlc1 in mtr10Δ strains. (A) The stability of mTlc1 did not depend on Mtr10 function. tlc1Δ MTR10 and tlc1Δ mtr10Δ strains carrying plasmids pmtr10-7 and pGAL-TLC1 were grown overnight at 18°C in galactose-containing medium. Cultures were shifted to 37°C for 2 h prior to addition of glucose. Samples were taken at the specified times, total RNA was extracted, and equal amounts of RNA were examined by Northern blot analysis. scR1 was probed as a loading reference. Signal intensities for Tlc1 were obtained with different exposures between strains. (B) mTlc1 fails to accumulate in mtr10Δ cells. Strains carrying pGAL-TLC1 were grown overnight (o/n) in raffinose-containing medium, then supplemented with galactose (2% final concentration), and sampled at the specified times. Equal amounts of total RNA from these samples were analyzed by Northern blotting. scR1 was used as a loading control. Samples from overnight-grown cells in galactose-containing medium are also shown (Gal).